A Simple Key For cytometer Unveiled

Info-enriched characterization of cell populations. Morphological data from illustrations or photos can incorporate towards the richness of flow cytometry knowledge. As an example, the figure exhibits an if not conventional apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in Every single population to expose morphologically unique attributes. These insights couldn't happen to be received from multiplex staining by yourself.

As samples are acquired on the Attune CytPix Flow Cytometer, the substantial-pace brightfield camera captures and retailers photos of detected activities at a charge as many as six,000 pictures for each second, with regards to the flow level and image measurement. For greater adaptability, Attune Cytometric Program enables you to adjust the picture capture frequency as required and choose a particular gate for graphic seize, if essential.

Merge acoustic focusing know-how with higher-parameter analysis for flexible spectral unmixing and standard workflows.

Discover new insights into your experimental information by visualizing populations utilizing blended fluorescence and morphology data. Our program interface wizards enable downsampling, concatenation (merged files) and dimensionality applications that are constructed in to the software for an intuitive, streamlined details Evaluation expertise.

"An image isn't quite truly worth a thousand dots, but it really truly helps you to see pictures. It is excellent to see photos that correlate with staining. By way of example, AnnexinV-optimistic cells which are morphologically various than wholesome cells, or CD14+ monocytes that happen to be much larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.

Furthermore, it can be illuminating to discover the quantity of doublets slip by classic singlet gates, and good to confirm the status of unpredicted double-constructive gatherings in my analysis."

Pair your Attune flow cytometer with an autosampler for increased workflow efficiency, and incorporate robotic integration for max throughput.

Constant graphic excellent even at significant throughput Acoustic focusing and a higher-pace camera Incorporate to image these T cells regularly at lower or large flow charges. Conveniently alter target and digicam options to fulfill experimental needs.

The Attune NxT cytometer would be the reputable workhorse instrument your lab can count on for consistent and trustworthy efficiency. The Attune CytPix design contains a higher-pace brightfield camera and AI-pushed impression Evaluation computer software, with the latest updates such as equipment Discovering tools and an intuitive user interface, enabling enhanced knowledge precision, precision, and speedier insights into sample biology.

End users can perspective occasion images in authentic-time to verify sample good quality, optimize protocols, and record the morphology of each cell inhabitants identified for Evaluation. Imaging seize also Added benefits from acoustic focusing, which helps to posture the cells in order that a sharp, centered image is attained.

AI-driven automatic impression Assessment Using the Attune Cytometric Application translates website celebration characteristics into unique morphology parameters that can be combined with common fluorescence and scatter parameters to expose hugely reproducible population variations and distinctive subsets.

Application Notice: Automatic impression Investigation reduces person-to-person variability in flow cytometry gating methods

Leverage the strength of Superior engineering and innovation to speed up your discoveries. Belief during the Attune Flow Cytometer family members to deliver the dependability, speed, and accuracy that set your get the job done apart.

Adaptable payment plan from Agilent Economical Methods let you get the vital instrumentation you would like for instant challenge, or to mature your lab.

Gating on these extended parameters means that you can speedily and correctly discover populations of interest and make sure gating strategy with little or no guide evaluation. The Attune computer software features usage of state-of-the-art equipment Mastering Assessment tools inside an expertly intended user interface, rendering it extremely easy to use.

The Attune Xenith cytometer is often a high-parameter spectral analyzer, giving equally spectral unmixing and standard flow capabilities for thorough and functional cell Assessment.

Agilent Benefit Guarantee guarantees you a minimum of ten years of instrument use from the date you buy. Usually, We'll credit history you Along with the residual price of the program towards an upgraded design.

Listed here, we demonstrate that by gating tactics employing image-centered quantitative parameters can distinguish interacting cells from coincident situations to much more correctly analyze interacting cells.